Structure · Laboratory

Plant Tissue Culture Laboratory

Haploid technologies for cereals, in vitro selection for resistance, embryo rescue in wide hybridisation, and microclonal propagation of plants.

Head of laboratory

Iryna S. Zambriborshch

PhD (Biology), Senior Researcher

izambriborsh@gmail.com +38 (067) 922-48-02 Google Scholar Web of Science

Crops in haploid technologies

NAAS programme tasks 2026–2030

Invention patents

Laboratory staff

Main research directions

Development and use of haploid technologies for cereals: soft winter wheat, durum winter wheat, rice, maize, barley, oats and rye.
Research on in vitro selection of cereals (wheat, barley, rice) based on the wide use of joint in vitro cultures (explant — pathogen) to evaluate and improve the efficiency of selecting resistant forms.
Embryo rescue technology in wide hybridisation of cereals.
Microclonal propagation of plants of various species.

NAAS programme tasks 2026–2030

NAAS research-programme tasks carried out by the laboratory in 2026–2030:

Development of a biotechnology for obtaining dihaploids of soft winter wheat through in vitro androgenesis using a liquid induction medium16.00.02.01.Ф

Creating an in vitro collection of cultivars of the perennial ornamental plant Heuchera L. for microclonal propagation16.00.02.05.П

The team at work

Publications & developments

Scientific publications6

Shestopal O. L., Chekalova M., Shpak D., Shpak T., Afinogenov A. Stabilisation of promising breeding rice plants resistant to Pyricularia oryzae. Zemdirbyste-Agriculture, 2023. 110 (1). P. 57–64.↗ source
Zambriborshch I., Shestopal O., Traskovetskaya V., Vasiliev O., Halaiev O., Halaieva M., Afinogenov O., Chekalova M. Obtaining dihaploid lines of winter bread wheat with complex resistance. Cereal Research Communications, 2024.↗ source
Zambriborshch I. S., Shestopal O. L., Chekalova M. S., Afinogenov O. A. In memory of Doctor of Biological Sciences, Professor Svitlana O. Ihnatova. Odesa National University Herald. Biology, 2024. 29(1(54)). P. 109–120. (in Ukrainian)↗ source
Zambriborshch I. S., Shestopal O. L., Chekalova M. S., Afinogenov O. A., Lytvynenko M. A., Vasyliev O. In vitro androgenesis in anther culture of soft winter wheat. Factors in Experimental Evolution of Organisms, 2024. Vol. 34. P. 149–153. (in Ukrainian)↗ source
Shestopal O., Zambriborshch I. Efficiency of in vitro androgenesis in anther culture of bread winter wheat (2021–2023). International Scientific Conference “Genetics, Physiology and Plant Breeding” (8th Edition): Materials Proceedings, Chisinau, October 7–8, 2024. P. 287–292.↗ source
Shestopal O. L., Zambriborshch I. S., Afinogenov O. A., Chekalova M. S., Vasyliev O. A., Traskovetska V. A., Sauliak N. I. Influence of iron source in induction medium on haplopreductive capacity of bread wheat in in vitro anther culture. Faktori eksperimental’noi evolucii organizmiv, 2025. Vol. 37. P. 193–198.↗ source

Methodical guidelines3

Improved technology of the soft winter wheat breeding process using biotechnological and molecular-genetic methods (co-author): scientific-methodical manual. Odesa: Astroprint, 2015. 41 p. (in Ukrainian)
Obtaining doubled haploids of rice (Oryza spp.) by in vitro anther culture: methodical guidelines / I. S. Zambriborshch, O. L. Shestopal, D. V. Shpak, M. S. Chekalova. Odesa, 2019. 13 p. (in Ukrainian)↓ PDF
Concept of creating rice (Oryza sativa L.) breeding material resistant to the blast pathogen (Pyricularia oryzae Br. & Cav.) using modern biotechnologies / D. V. Shpak, I. S. Zambriborshch, D. P. Palamarchuk, T. M. Shpak, O. L. Shestopal, H. V. Melnichenko, M. S. Chekalova, O. A. Afinogenov; PBGI NCSCI. Odesa: Astroprint, 2022. 23 p. (in Ukrainian)↓ PDF

Invention patents6

UA 108514 U · No. 147115 · 2021
Selection of donor plants to increase the efficiency of the biotechnology for obtaining line material of soft winter wheat at the first stages of breeding selection
UA 108514 U · No. u201512317 · 2016
Method for obtaining rice lines
UA 108512 U · No. u201512315 · 2016
Nutrient medium C17B for inducing neoplasms in in vitro anther culture of durum wheat
UA 108514 U · No. u201512316 · 2016
Method for obtaining neoplasms in anther culture of winter rye (co-author)
No. u200611658 · 2007
Method for increasing regeneration potential in anther culture of soft winter wheat
No. 13641 · IPC (2006) A01H 102 · 2006
Method for overcoming progamic and postgamic incompatibility barriers in wide hybridisation of soft wheat with Aegilops tauschii Coss.

Plant variety. Soft winter wheat variety Moment with group disease resistance, var. ErythrospermumApplication No. 2025012018 of 28.03.2025 (Ministry of Economy, Environment and Agriculture of Ukraine).